POD reacting with hydrogen peroxide (H2O2) catalyzes oxidative condensation of tyramide to create extremely reactive intermediates, tyramide radicals. The activated tyramide covalently binds to electron-wealthy moieties like tyrosine, phenylalanine and tryptophan at or close to the POD. The shorter 50 %-life of the tyramide radicals restricts the deposition of tyramide3. https://buckminsterh655wiw9.eveowiki.com/user